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PURITY ANALYSIS METHOD OF 1-METHYL-3,5-DINITRO-1H-1,2,4-TRIAZOLE

Jin Luo, Yu-cun Liu, Yan Liu, Guo-Dong Wang, Jian-hua Wang, Jun-Ming Yuan
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Abstract


A HPLC method was established to analyze main ingredient – 1-methyl-3,5-dinitro-1H-1,2,4-triazole and the main impurity – 1-methyl-3-nitro-1H-1,2,4-triazol-5-amine. The method was carried out on a SinoChrom ODS-BP column (4.6 × 200 mm, 5 μm) using methanol–water (90/10, v/v) as the mobile phase at a flow rate of 1.0 ml·min–1. 1-Methyl-3,5-dinitro-1H-1,2,4-triazole was determined by UV-visible detector at 240 nm. The column temperature was 25°C and the injection volume was 10 μl. Results show that under the optimized chromatographic conditions, the calibration curves of 1-methyl-3,5-dinitro-1H-1,2,4-triazole and 1-methyl-3-nitro-1H-1,2,4-tiazol-5-amine have good linearity, and the linear correlation coefficient r is greater than 0.999. The detection limits for 1-methyl-3,5-dinitro-1H-1,2,4-triazole and 1-methyl-3-nitro-1H-1,2,4-triazol-5-amine were 0.6 and 0.31 mg·ml–1, respectively, and the quantification limits were 1.02 and 0.53 mg·ml–1, respectively. The recoveries are 98.17–100.83% with the relative standard deviations between 0.44 and 1.01%. The method has wide linear range, high sensitivity, and good reproducibility, and the results are accurate and reliable.

Keywords


1-methyl-3,5-dinitro-1H-1,2,4-triazole; 1-methyl-3-nitro-1H-1,2,4-triazol-5-amine; analytical chemistry; HPLC; purity analysis

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