CYTOTOXIC AND APOPTOTIC ACTIVITY OF NITROFUROXANS ON LYMPHOMA CELLS

Authors

  • Sergey N Kolomeichuk Institute of Biology Karelian Research Center Russian Academy of Sciences
  • Yakov P Nizhnik Bioo Scientific Corporation, Austin, TX
  • Nina N Makhova N.D. Zelinsky Institute of Organic Chemistry RAS, Moscow
  • Igor Ovchinnikov N.D. Zelinsky Institute of Organic Chemistry RAS, Moscow

DOI:

https://doi.org/10.1007/4254

Keywords:

nitrofuroxans, apoptosis, cytotoxicity, lymphoma cell lines.

Abstract

Five nitrofuroxans were synthesized and evaluated for their in vitro cytotoxicity against human lymphoma cells with different p53 status (Raji Burkitt's lymphoma and MOLT-4). The most promising compound exhibited IC50 values of 2.6–11.0 μM in MTT growth inhibitory assays. Flow cytometry data suggest that cytotoxic effects of given compounds in MOLT-4 cells are mediated by apoptosis via a p53-dependent pathway. Changes in nuclear morphology after treatment were evaluated by fluorescent microscopy after Hoechst staining. However, the studied nitrofuroxans did not induce apoptosis in Raji cells having the p53 mutant gene, thus suggesting a different mechanism of their action.

 

Author Biography

Sergey N Kolomeichuk, Institute of Biology Karelian Research Center Russian Academy of Sciences

Dept of genetics

Senior research associate

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Published

2018-02-16

Issue

Vol. 54 No. 1 (2018)

Section

Original Papers